O interactions help to form chains of molecules in the crystal structure.
With the exception of the methylene H atoms, all atoms lie on a mirror plane. O hydrogen bonds are observed. The are two molecules in the asymmetric unit.
Here, protocols for a simple, fast and affordable method for transient protein _expression_ in mammalian cells are reported. SPINE was able to bring together developers of software with users from the partner laboratories.
Papers on the crystallization of biological molecules will be accepted providing that these focus on new methods or other features that are of general importance or applicability. Therefore, screening for soluble _expression_ on a small scale is an attractive way of identifying constructs that are likely to be amenable to structural analysis. Surprisingly, this could not be overcome by modifications of the constructs, changes of _expression_ temperature or strain or renaturation. N hydrogen bonds, leading to a chain structure.
Often, this is caused by the poor quality of the protein preparation used for crystallization trials. O hydrogen bonds, linking the independent molecules into dimers, may be effective in the stabilization of the crystal structure.
Some individual projects and developments are discussed in detail, while those that are covered elsewhere in this issue are treated more briefly.
Although isolation and structure determination of endogenous complexes has been reported, much progress has to be made to make this technology easily accessible.
The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations.
O hydrogen bonds are observed.
Target analysis by biophysical clustering based on pI and hydropathy has provided useful information for future target-selection strategies.
Papers on crystallographic methods should be oriented towards biological crystallography, and may include new approaches to any aspect of structure determination or analysis. Finally, a forward view is taken of how crystallization methodologies may develop in the future.
Papers on the crystallization of biological molecules will be accepted providing that these focus on new methods or other features that are of general importance or applicability. With the exception of the methylene H atoms, all atoms lie on a mirror plane.
However, prokaryotic based _expression_ systems fail to generate correctly folded functional forms of many proteins and hence a variety of eukaryotic based _expression_ systems have been developed. The other two N atoms are each linked to an O atom by methylene groups, forming a six-membered ring.